Figure 2From: Transcriptional implications of intragenic DNA methylation in the oestrogen receptor alpha gene in breast cancer cells and tissuesThe effect of decitabine on DNA methylation and expression in breast cancer cell lines. A) Composite graphs showing the DNA methylation levels across the ESR1 gene in three ER-neg cell lines treated with DAC (yellow) compared to DMSO-treated controls (red). Data was collected in triplicate in each cell line at each locus, and the standard error of the mean was then calculated (error bars); *p < 0.05, Bonferroni corrected t-test. B) DNA methylation across the ESR1 gene in ER-pos cell lines treated with DAC (purple) compared to untreated (blue). C, D) qRT-PCR gene expression in individual cell lines treated with DAC (ER-neg = yellow, ER-pos = purple) compared to DMSO-treated controls (ER-neg = red; ER-pos = blue). Expression increased significantly in ER-neg cell lines, but was significantly reduced in all three ER-pos cell lines (p < 0.05).Back to article page