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Table 3 Validation of the three assays used

From: Massively parallel sequencing fails to detect minor resistant subclones in tissue samples prior to tyrosine kinase inhibitor therapy

Assay

Sensitivity

Specificity

Limit of detection

GS Junior

100% (21/21)

100% (69/69)

1%#

MiSeq™ - Qiagen panel

100% (77/77)

100% (118/118)

0.03 – 0.25%#

MiSeq™ - AmpliSeq panel

93% (69/74)

100% (83/83)

0.02 – 0.45%#

  1. Shown are the sensitivity, specificity and limit of detection for each assay.
  2. Sensitivity: Proportion of correctly identified positive events (True positive rate).
  3. Specificity: Proportion of correctly identified negative events (True negative rate).
  4. (/): (number of detected/number of expected events).
  5. #See Additional file 5 for detailed information.