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Table 3 Validation of the three assays used

From: Massively parallel sequencing fails to detect minor resistant subclones in tissue samples prior to tyrosine kinase inhibitor therapy

Assay Sensitivity Specificity Limit of detection
GS Junior 100% (21/21) 100% (69/69) 1%#
MiSeq™ - Qiagen panel 100% (77/77) 100% (118/118) 0.03 – 0.25%#
MiSeq™ - AmpliSeq panel 93% (69/74) 100% (83/83) 0.02 – 0.45%#
  1. Shown are the sensitivity, specificity and limit of detection for each assay.
  2. Sensitivity: Proportion of correctly identified positive events (True positive rate).
  3. Specificity: Proportion of correctly identified negative events (True negative rate).
  4. (/): (number of detected/number of expected events).
  5. #See Additional file 5 for detailed information.