Table 1 Oligonucleotide primer sequences for PCR or Reverse transcription

Primers for reverse transcription

miR-124

5′-GTCGTATCCAGTGCAGGGTCCG AGGTATTCGCACTGGATACGA

CGGCATTC-3′

U6

5′-AAAATATGGAACGCTTCACGAATTTG-3′

STAT3

5′-TTTTTTTTTTTTTTTTTT-3′(Oligo(dT))

GAPDH

5′-TTTTTTTTTTTTTTTTTT-3′(Oligo(dT))

Primers for quantitative real-time PCR

miR-124-F

5′- GGACTTTCTTCATTCACACCG-3′

miR-124-R

5′- GACCACTGAGGTTAGAGCCA-3′

U6-F

5′-CTCGCTTCGGCAGCACATATACT-3′

U6-R

5′-ACGCTTCACGAATTTGCGTGTC-3′

STAT3-F

5′-GAAGGACATCAGCGGTAAGA-3′

STAT3-R

5′-AGATAGACCAGTGGAGACAC-3′

GAPDH-F

5′-TCAACGACCACTTTGTCAAGCTCA-3′

GAPDH-R

5′-GCTGGTGGTCCAGGGGTCTTACT-3′

Primers for STAT3 PCR amplifying

Up-stream

5′-TGACTCCCTTTCTCCCTGG-3′

Down-stream

5′-GAACTGAATGAAGACGCCAT-3′