Skip to main content

Table 1 GEP DNA copy number with reference to A.) centromere 17 (CEN17) 1 and B.) centromere 3 (CEN3) 2 by FISH analysis 3,4

From: Copy number gain of granulin-epithelin precursor (GEP) at chromosome 17q21 associates with overexpression in human liver cancer

A.

GEP

CEN17

GEP

CEN17

HCC

RP11-436 J4

pEZ17-4

RP11-52 N13

pEZ17-4

801

3.66

3.37

3.80

3.51

884

3.08

2.92

3.02

2.77

B.

GEP

CEN3

GEP

CEN3

HCC

RP11-436 J4

pAE0.68

RP11-52 N13

pAE0.68

801

3.73

2.17

3.73

1.97

884

2.85

2.10

2.89

1.92

  1. 1GEP DNA copy number with reference to centromere 17 (CEN17). In these two cases, CEN17 scores ranged 2.92 to 3.51 per cell, and similarly GEP scores ranged 3.02 to 3.80 per cell. The data indicated an increased chromosome 17 copy number, at centromere and GEP locus at 17q21, in these cases.
  2. 2GEP DNA copy number with reference to centromere 3 (CEN3). CEN3 scores ranged 1.92-2.17 per cell, indicating approximately two copies of chromosome 3. The number of GEP DNA per 2 centromere (diploid) was 3.60 and 2.86 for HCC801 and HCC884, respectively, by FISH analysis. GEP copy number was 2.56 and 2.93 for HCC801 and HCC884, respectively, by QuMA (reference to D3S1609).
  3. 3DNA copy number for each set was quantified for 100 cells and data presented per cell.
  4. 4Noted independent platform using QuMA qPCR, both HCC801 and HCC884 showed similar increased GEP DNA copy number.