Skip to main content
Figure 5 | BMC Cancer

Figure 5

From: The natural compound Guttiferone F sensitizes prostate cancer to starvation induced apoptosis via calcium and JNK elevation

Figure 5

GF affected Bcl-2 family, MAPKs, and androgen receptor (AR). LNCaP cells were treated with 10 μM GF for 6, 12 and 24 h, respectively. (A) Western blot showed the expression of Bcl-2, Bax and Bcl-xL in LNCaP cells treated with 10 μM GF with or without FBS. (B) The ratio of Bcl-2/Bax protein expression. (C) Relative Bcl-2 and PUMA mRNA level (compared with GAPDH) was analyzed by quantitative RT-PCR. LNCaP cells were treated for 0 h as control. Cells were treated with GF (10 μM) in serum-free medium or serum medium for 6 h. * indicates a significant difference from the controls. p < 0.05. (D) The protein expression of MAPKs and AR, phosphorylated ERK and phosphorylated ERK in LNCaP cells treated with 10 μM GF with or without FBS.

Back to article page