Curcumin potentiates 5-FU to inhibit migration of HCT116 and HCT116R cells in alginate beads. Quantification of the spheroid numbers emigrated through alginate beads after 14 days in culture. The cultures of HCT116 and HCT116R cells were treated as described above (A-C) and evaluated by Toluidine blue staining. Concentrations of curcumin or/and 5-FU resulting in 50% invasion inhibition were indicated as individual IC50 values. The results are provided as mean values with standard deviations from at least three independent experiments. Values were compared with the control and statistically significant values with p < 0.05 were designated by an asterisk (*) and p < 0.01 were designated by two asterisks (**).