Expression of CXCR4, MMP9 and NF-κB p65, in HCT116 (a) and HCT116R (b) cells. Cells encapsulated in alginate beads (A) compared with migrated (invaded) (I) and adhered (Ad) cells after 1, 7, 14, 21 and 28 days of culture as shown by western blotting evaluation and was confirmed by quantitative densitometry. Western blots shown are representative of three independent experiments. The housekeeping protein β-actin served as a positive loading control in all experiments. Values were compared with the control and statistically significant values with p < 0.05 were designated by an asterisk (*) and p < 0.01 were designated by two asterisks (**).