Association of AF-6/afadin expression with histological grade and myometrial invasion in 85 endometrioid adenocarcinomas. (a) The staining values of 0, 1, 2, or 3 are negative, weak, moderate, or strong, respectively. (b, c) Low H-score of AF-6/afadin immunoreactivity was associated with high histological grade (between grade 1 and 2 cases, and between grade 1 and 3 cases, P < 0.01, and 0.01, respectively) and deep myometrial invasion (between no invasion and more than half invasion cases, P < 0.01) (b). The correlations between AF-6/afadin H-score and histological grade or myometrial invasion were assessed with the Kruskal-Wallis H-test; the Mann–Whitney U-test with Bonfferoni correction was used as a post hoc test. Significant differences were indicated as * for P < 0.05 and ** for P < 0.01.