Figure 3

Immunoblot analyses of urine samples from BPH and prostate cancer patients. Representative urine samples were separated on 4-12% Bis-Tris gels under reducing conditions and were subsequently subjected to western blot analysis using the appropriate antibody for the following iTRAQ-identified proteins: mucin 3 fragment (MUC3 25 kDa), mucin 3 fragment (MUC3 51 kDa), β-2-microglobulin (β2M), pepsinogen 3 (PGA3), apoliprotein D (ApoD), alpha-2-glycoprotein 1, zinc (ZAG) and uromodulin (THP). One hundred seventy-three (173) urine samples from patients diagnosed with benign disease (N = 83) and tumor disease (N = 90) were analyzed.