Skip to main content

Table 1 Gene expression in vascular fragments

From: Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts

Gene product

Shb+/+

Shb+/−

VEGFA

1.0 ± 0.12

1.1 ± 0.15

PlGF

1.0 ± 0.25

1.8 ± 0.22 *

PDGF-AA

1.0 ± 0.15

1.2 ± 0.11

PDGF-BB

1.0 ± 0.21

1.2 ± 0.32

PDGF-CC

1.0 ± 0.09

1.0 ± 0.08

PDGF-DD

1.0 ± 0.64

1.3 ± 0.22

Angiopoietin-1

1.0 ± 0.13

1.1 ± 0.25

Angiopoietin-2

1.0 ± 0.22

1.0 ± 0.34

VEGFR1

1.0 ± 0.16

1.0 ± 0.28

VEGFR2

1.0 ± 0.18

1.1 ± 0.27

Tie-2

1.0 ± 0.22

1.2 ± 0.31

PDGFRA

1.0 ± 0.11

0.7 ± 0.09 *

PDGFRB

1.0 ± 0.27

1.0 ± 0.30

GM-CSF

1.0 ± 0.11

0.8 ± 0.40

CXCL-12

1.0 ± 0.59

0.9 ± 0.22

CXCR-4

1.0 ± 0.46

1.3 ± 0.22

CXCR-7

1.0 ± 0.63

1.4 ± 0.36

  1. RNA isolated from vascular fragments was analyzed by real-time RT-PCR. Ct values were determined using the Roche Lightcycler software and beta-actin values were subtracted to normalize for differences in total amounts of RNA. Relative values shown in table were calculated by the formula 2-ΔCt (Shb+/− minus Shb+/+). *indicates p < 0.05 when compared with wild type. N = 5–7.
Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us