Table 1 Antibodies used for immunohistochemical analyses
Proteins | Manufacturer | Dilution | Positive |
|---|---|---|---|
TLR3 | ABCAM (AB12085) | 1:50 | Membrane, Cytoplasm |
TRIF | ABCAM (AB101232) | 1:50 | Membrane, Cytoplasm |
NF-κB | Cell signaling (SER536) | 1:100 | Cytoplasm, Nucleus |
IRF3 | ABCAM (AB76493) | 1:50 | Cytoplasm, Nucleus |
Ki67 | Fuzhou Maixin Biotech (RMA-5542) | 1:50 | Nucleus |
CyclinD1 | Fuzhou Maixin Biotech (RMA-0541) | 1:50 | Nucleus |
Survivin | Fuzhou Maixin Biotech (RAB-0536) | 1:50 | Cytoplasm |
Bcl-2 | ABCAM (AB12085) | 1:100 | Cytoplasm |
caspase 3 | Cell signaling (ASP175) | 1:50 | Cytoplasm |
Caspase 8 | Cell signaling (ASP391) | 1:50 | Cytoplasm |
Caspase 9 | Proteintech (10380-1-AP) | 1:50 | Cytoplasm |
CD34 | Fuzhou Maixin Biotech (MAB-0034) | 1:50 | Membrane, Cytoplasm |
MMP-2 | Fuzhou Maixin Biotech (MAB-0244) | 1:50 | Cytoplasm |
Two-step immunohistochemical staining kit | Fuzhou Maixin Biotech (KIT-9801) | Â | |