Figure 6

SRSF4 contributes to cisplatin-induced cell death. A-D: Apoptosis was measured by FACS after annexin V/propidium iodide staining of siSCR (A,B) or siSRSF4 (C,D) transfected MCF-7 cells untreated (A,C) or treated with cisplatin (B,D; 50 μM, 24 hours). The percentages in each quartile are mean values calculated from three independent experiments. E. Quantification of data from trypan blue exclusion and FACS analysis. Histograms indicate cell death in siSRSF4 transfected MCF7 cells treated with cisplatin as compared to death in cells transfected with control siRNA (siSCR) taken as 100%. Data were corrected for cell death measured in untreated cells. Each bar shows the mean with SD of three independent experiments.