Figure 1

Establishment of cellular resistance models. Bladder cancer cell lines 5637 and RT4 were adapted to growth in the chemotherapeutic presence of 20 ng/ml gemcitabine (^rGEMCI²⁰) or 1000 ng/ml Cisplatin (^rCDDP¹⁰⁰⁰). 50% inhibiting concentration (IC₅₀) Values are mean from three independant experiments ± SD and are shown in A. Chemo-sensitive (5637, RT4) and chemo-resistant cells (5637^rGEMCI²⁰, 5637^rCDDP¹⁰⁰⁰, RT4^rGEMCI²⁰, RT4^rCDDP¹⁰⁰⁰) were treated with 3 μM Staurosporin (STS), an inducer of apoptotic cell death, for 6 h and cell viability was measured by the MTT Assay.*, P < 0.05 compared with the parental cell line (B).