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Figure 2 | BMC Cancer

Figure 2

From: Direct confirmation of quiescence of CD34+CD38- leukemia stem cell populations using single cell culture, their molecular signature and clinicopathological implications

Figure 2

Comparison of plating efficiency and proliferation capacities in single normal HSCs and ASCs. The growth and proliferation capacities of single HSCs and ASCs were quantified and graded with the following scoring system according to the number of cells in each well of 96 microplate: Grade 1, ≤5 cells/well; Grade 2, 6 to 10 cells/well; Grade 3, 11 to 20 cells/well; and Grade 4, ≥21 cells/well. (A) The plating efficiency of single ASC was significantly lower than that of normal HSCs obtained from cord blood (CB), peripheral blood (PB), and adult bone marrow (BM). The plating efficiency of single HSCs varied among source samples. Values for plating efficiency of ASCs isolated from BM obtained from 7 AML patients are indicated with black circles; while those of normal HSCs in BM and PB obtained from healthy controls (n = 6) are indicated with white circles; and those for CB obtained from healthy controls (n = 5) are indicated with gray circles. (B) Almost all single CB cells showed high proliferative capacity of Grade 4; BM and PB stem cells showed similar grades of single cell plating efficiency. However, almost all single ASCs showed significantly lower dividing property at Grade 1. Statistical significance is indicated as follows: *, p-value < 0.05 and **, p-value < 0.01.

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