Figure 4
From: Topotecan synergizes with CHEK1 (CHK1) inhibitor to induce apoptosis in ovarian cancer cells
Simultaneous treatment with TPT and PF477736 was generally more potent than sequential treatments of individual drugs. Treatment schedules of 24 (A) and 48 (B) hours apart between drugs are shown. The concentrations of TPT and PF477736 were 10 nM and 0.1 μM, respectively, except Ovcar5 (0.3 μM PF477736). Cellular viability was measured by XTT assay in 3 replicates and calculated relative to untreated cells.