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Figure 5 | BMC Cancer

Figure 5

From: Oridonin stabilizes retinoic acid receptor alpha through ROS-activated NF-κB signaling

Figure 5

Oridonin-induced RARα stability requires the activation and nuclear translocation of p65. (A) NB4/GFP and NB4/GFP-MAD cells were treated with 10 μM oridonin for 12 h. The intracellular localization of p65 was analyzed using anti-p65 antibodies (red) with DAPI staining (blue) for nuclei. Scale bars represent 20 μm. (B, C) NB4/GFP and NB4/GFP-MAD cells were treated with 10 μM oridonin for 12 h (B) or treated with 5 μM H2O2 for the times indicated (C), and the cell lysates were western blotted for the indicated proteins. (D) NB4 cells were infected with pSIREN-RetroQ-derived retroviruses carrying shRNA for p65 or scrambled shRNA as a control, and the cell lysates were western blotted for the indicated proteins. (E, F) NB4-NC and NB4-sh-p65 cells were treated with 10 μM oridonin for 12 h (E) or with 5 μM H2O2 for the times indicated (F), and the cell lysates were western blotted for proteins as indicated. All experiments were repeated three times and gave consistent results.

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