Figure 3

Validation of SRM-based results and correlated expression of S100 proteins with ANXA1 and ANXA2. a) Western blot of S100A13 showing overexpression in PTC tissues; b) Western Blot of S100A13 (S100A13/Rab7a, one PTC outlier excluded) showed agreement with SRM-based results; c) Western blot of S100A4, β-tubulin and Rab7a in thyroid tumours and normal thyroid . β-tubulin presented higher variation (CV = 46%) than Rab7a (CV = 19%); d) Normalised S100A4 expression (S100A4/Rab7a) was well correlated with that from SRM analysis. e) Expression of S100A11 and its interaction partner ANXA1 are highly correlated across all samples, and are overexpressed in PTC; f) Expression of S100A10 and its interaction partner ANXA2 are also highly correlated across all samples, and are both reduced in FA and FTC.