Figure 5
MTUS1/ATIP3a overexpression is related to the migration and invasion of SACC. (A) Differential expression of ERK-Slug pathway in SACC-LM cells treated with either control vector or ATIP3a plasmid. SACC-LM cells displayed decreased Slug, Vimentin and pERK1/2 protein levels and increased E-cadherin, ATIP3a protein levels upon ATIP3a overexpression. ATIP3a protein was detected by anti-MTUS1 antibody. (B and C) The migration and invasion ability of SACC cells was assessed by a transwell migration and invasion assay. ATIP3a overexpression significantly inhibited the migration (B) and invasion (C) of SACC-LM cells. (D) Cell proliferation was measured using a MTT assay. The cell proliferation rate of SACC-LM was significantly inhibited after overexpressed with MTUS1/ATIP3a. *: P < 0.05.