Figure 7
CXCR7 modulates androgen-mediated cell motility through CXCR4. (A) Transwell assay assessing the effects of androgens on LNCaP migration. ANOVA was used to determine significant differences between vehicle (ethanol) and androgen (R1881) treated cells (*p ≤ 0.05, n = 3). (B-D) Western blot analysis of LNCaP membrane glycoproteins enriched from cells grown in androgen-depleted medium for 72 hrs and treated with 0, 0.1, 1, or 10 nM R1881 for 24 hrs with antibodies to (B) PSA, PSMA, (C) CXCR7, and (D) CXCR4. Red asterisks = putative glycosylated CXCR4 and CXCR7 isoforms. The densitometry values were normalized to vehicle-treated lysates and labeled below the blots. (E) Silver staining demonstrates equivalent loading across the samples. (F) Immunofluorescence staining of CXCR4 and CXCR7 in semi-permeabilized AD-LNCaP cells treated with 0, 0.1, 1, or 10 nM R1881. The nuclei are labeled with DAPI.