Figure 4

CXCR7 functionally interacts and colocalizes with AR. (A) Western blot of LNCaP cells transfected with the indicated siRNA combinations: control (50 nM), AR (50 nM), CXCR7 (50 nM), AR/control (25 nM/25 nM), CXCR7/control (25 nM/25 nM), or AR/CXCR7 (25 nM/25 nM) for 72 hrs. Western blot was performed using AR, CXCR7, and PSA antibodies. Silver staining demonstrates equivalent loading across the samples. The densitometry values were normalized to control siRNA transfected cells and labeled below the blots. (B) Immunofluorescence analysis of CXCR7 and AR in LNCaP cells under AR or CXCR7 knockdown conditions. Cells were transfected with control (I-I to I-III), AR (II-I to II-III), or CXCR7 (III-I to III-III) siRNA, stained with antibodies against AR and CXCR7, and treated with DAPI.