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Figure 2 | BMC Cancer

Figure 2

From: Androgen receptor and chemokine receptors 4 and 7 form a signaling axis to regulate CXCL12-dependent cellular motility

Figure 2

CXCR7 subcellular localization in prostate-cancer cells. (A) Western blot of proteins from cytosolic, membrane, nuclear, and chromatin fractions isolated from normal, AD-, and AS-LNCaP cells with antibodies to pAbCXCR7 (top left panel), Hsp90, EEA1, AR, or histone H3 (right panels, with blank lanes in lanes 5 and 10). Silver-stained gel demonstrated equal protein loading across samples (bottom left panel). (B) Upper panels: AD-LNCaP cells. Left panel: Image of a single optical section (section 17 out of 32), with “Cut View” analysis of the entire Z-stack shown in margins. Green = CXCR7, Red = EEA1, Blue = DAPI. Right top panel: Reconstruction of entire Z-stack of sample shown in left. Scale bar = 20 μm. Right bottom panel: Similar reconstruction of cells stained in the presence of competing peptide. Scale bar in lower right = 20 μm and is for top and bottom right panels. Lower panels: Androgen stimulated LNCaP cells. Left panel: Image of a single optical section (section 12 out of 32), with “Cut View” analysis of the entire Z-stack shown in margins. Green = CXCR7, Red = EEA1, Blue = DAPI. Scale bar = 20 μm. Right top panel: Reconstruction of entire Z-stack of sample shown in left. Right bottom panel: Similar reconstruction of cells stained in the presence of competing peptide. Scale bar in lower right = 20 μm and is for top and bottom right panels.

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