Figure 1
From: The role of multipotent cancer associated fibroblasts in hepatocarcinogenesis
The phenotypes of primary cells CAF and NTF. A. Flow cytometric analysis of surface marker proteins showed the positivity for CD90 and CD44, and the negativity for CD133, CD45, and STRO-1. Black graph: control, White graph: positive expression. B. Morphology of the cells and localization of positive proteins CD90, CD44, αSMA, and Vimentin. Scale bar, 100 μm. C. mRNA expressions of vimentin (VIM) and αSMA (ACTA2) in CAF and NTF. VIM and ACTA2 mRNA relative expressions were normalized to reference genes RNA18S and ACTB. D. 3D clonogenic capacity of the CAF in Matrigel. Scale bar, 100 μm.