Figure 2From: Combined treatment of Nimotuzumab and rapamycin is effective against temozolomide-resistant human gliomas regardless of the EGFR mutation statusNimotuzumab and rapamycin combination treatment enhanced therapeutic efficacy in Asian Glioma cell lines. (A) Western blot analysis was done to check the EGFR expression status in G5T/VGH, GBM8401 and GBM8901 Asian glioma cell lines with respective (+) controls. These cell lines were treated with TMZ (500 μM), rapamycin (0.1 mM) or Nimotuzumab (0.013 mM) as single treatments or combination of rapamycin and Nimotuzumab for 24 h and percentage of cell viabilities of G5T/VGH (B), GBM8401 (C) and GBM8901 (D) were determined. Data are presented as mean ± SEM. Combination groups were compared to each single drug treatments in individual cell line *p < 0.05, ***p < 0.001.Back to article page