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Table 4 Dot blot analysis of the activation of MAP kinase family members in SW1736, HTh7, BHT101 and ML1 cells after short-term treatment (10 min) with 3 μM sorafenib

From: Sorafenib inhibits intracellular signaling pathways and induces cell cycle arrest and cell death in thyroid carcinoma cells irrespective of histological origin or BRAF mutational status

 

% of untreated control

Protein

SW1736

HTh7

BHT101

ML1

p-ERK1

54.2 ± 8.1*

67.1 ± 4.5*

45.4 ± 9.2*

82.0 ± 6.0*

p-ERK2

76.0 ± 5.4*

73.7 ± 3.7*

84.1 ± 5.7

88.1 ± 7.9

p-p38 alpha

38.9 ± 8.4*

44.4 ± 6.0*

32.2 ± 8.9*

61.3 ± 6.6*

p-p38 beta

68.6 ± 6.2*

80.7 ± 2.8*

75.9 ± 6.3*

76.7 ± 7.1*

p-p38 gamma

64.7 ± 5.9*

75.2 ± 5.4*

49.3 ± 5.7*

49.5 ± 8.3*

p-p38 delta

78.4 ± 5.0*

82.8 ± 8.8

83.6 ± 8.4

89.3 ± 6.9

p-JNK1

89.0 ± 7.7

79.1 ± 5.7*

86.7 ± 7.9

73.0 ± 9.8*

p-JNK2

61.2 ± 7.1*

75.1 ± 6.2*

77.5 ± 7.0*

63.2 ± 6.2*

p-JNK3

103.5 ± 7.7

86.3 ± 8.6

89.6 ± 5.4

69.2 ± 6.8*

p-AKT1

72.1 ± 5.9*

80.2 ± 5.8*

83.4 ± 6.3*

75.7 ± 5.5*

p-AKT2

74.7 ± 9.2*

80.0 ± 7.2*

81.9 ± 7.0*

77.4 ± 6.1*

p-AKT3

92.3 ± 8.3

77.6 ± 6.2*

81.5 ± 5.6*

86.6 ± 9.7

  1. Abbreviations: ERK extracellular-signal regulated kinase, p38 p38 mitogen-activated kinase, JNK c-jun N-terminal kinase, AKT AKT/protein kinase B.
  2. Values for the respective protein compared to the vehicle-treated control ± standard deviation are depicted and represent 6-fold determinations. *indicates significant decrease (p<0.05, Student’s t-test).