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Table 4 Dot blot analysis of the activation of MAP kinase family members in SW1736, HTh7, BHT101 and ML1 cells after short-term treatment (10 min) with 3 μM sorafenib

From: Sorafenib inhibits intracellular signaling pathways and induces cell cycle arrest and cell death in thyroid carcinoma cells irrespective of histological origin or BRAF mutational status

  % of untreated control
Protein SW1736 HTh7 BHT101 ML1
p-ERK1 54.2 ± 8.1* 67.1 ± 4.5* 45.4 ± 9.2* 82.0 ± 6.0*
p-ERK2 76.0 ± 5.4* 73.7 ± 3.7* 84.1 ± 5.7 88.1 ± 7.9
p-p38 alpha 38.9 ± 8.4* 44.4 ± 6.0* 32.2 ± 8.9* 61.3 ± 6.6*
p-p38 beta 68.6 ± 6.2* 80.7 ± 2.8* 75.9 ± 6.3* 76.7 ± 7.1*
p-p38 gamma 64.7 ± 5.9* 75.2 ± 5.4* 49.3 ± 5.7* 49.5 ± 8.3*
p-p38 delta 78.4 ± 5.0* 82.8 ± 8.8 83.6 ± 8.4 89.3 ± 6.9
p-JNK1 89.0 ± 7.7 79.1 ± 5.7* 86.7 ± 7.9 73.0 ± 9.8*
p-JNK2 61.2 ± 7.1* 75.1 ± 6.2* 77.5 ± 7.0* 63.2 ± 6.2*
p-JNK3 103.5 ± 7.7 86.3 ± 8.6 89.6 ± 5.4 69.2 ± 6.8*
p-AKT1 72.1 ± 5.9* 80.2 ± 5.8* 83.4 ± 6.3* 75.7 ± 5.5*
p-AKT2 74.7 ± 9.2* 80.0 ± 7.2* 81.9 ± 7.0* 77.4 ± 6.1*
p-AKT3 92.3 ± 8.3 77.6 ± 6.2* 81.5 ± 5.6* 86.6 ± 9.7
  1. Abbreviations: ERK extracellular-signal regulated kinase, p38 p38 mitogen-activated kinase, JNK c-jun N-terminal kinase, AKT AKT/protein kinase B.
  2. Values for the respective protein compared to the vehicle-treated control ± standard deviation are depicted and represent 6-fold determinations. *indicates significant decrease (p<0.05, Student’s t-test).
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BMC Cancer

ISSN: 1471-2407

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