Figure 5

LIF mediates the TGFβ-dependent inhibition of melanoma cell migration. A, WM278 cells treated with LIF (100 or 500 ng/mL) or B, transfected with a scrambled or a LIF or a p21 siRNA 48 h earlier were plated in starvation medium on top of 24-well cell culture Transwell inserts, stimulated or not with TGFβ for 48 h, and the migratory cells were labeled with crystal violet, photographed using phase contrast light microscopy. A representative image is shown for each cell line (left panel). Migratory cells were counted using the ImageJ software and graphed (right panel). Data is graphed as the mean of at least 3 biological replicates. Error bars are the standard errors of the mean. For statistical analysis the t-test was performed compared to the non-treated mock condition (*p < 0.05).