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Figure 5 | BMC Cancer

Figure 5

From: Overexpression of 17β-hydroxysteroid dehydrogenase type 10 increases pheochromocytoma cell growth and resistance to cell death

Figure 5

Effect of HSD10 overexpression on cellular resistance to death-inducing stimuli. A-B. Densitometry of MTT reduction in EV and HSD10 ov cells treated with A) 0, 0.1, 0.25, 0.5, 0.75, and 1 mM H2O2 (n = 3 for all groups), and B) 0, 0.1, and 0.25 mM TBH for 24 hours (n = 3 for all groups). Results demonstrated that HSD10 ov cells were more resistant to oxidative stress-induced cell death. C-D. ETC. complex IV enzyme activity was assessed in EV and HSD10 ov cells treated with 0.75 mM H2O2 for C) 24 hours (n = 3), and D) 0, 1, 6, and 16 hours (n = 6 for all time points). Results, displayed as nmol/mg protein/min/ml, showed that complex IV activity is enhanced in HSD10 ov cells under an oxidative stress condition. E. Confocal microscopy demonstrating TUNEL staining of cells undergoing apoptosis (green), nuclear staining with DAPI (blue), and these two antigens co-localized (merged) in EV and HSD10 ov cells, treated with 0 mM and 0.75 mM H2O2 for 24 hours. Scale bar in E: 30 μm. F. Quantification of TUNEL staining (depicted in E) displayed as the percentage of TUNEL positive cells (n = 4). Data presented as mean ± SE. *P < 0.05, **P < 0.01, ***P < 0.0001 versus EV control group (A-D) and versus EV and HSD10 ov non-treatment groups (F); #P < 0.01 versus EV treatment group (F).

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