Effect of HSD10-modification onin vitrocell growth andin vivotumor growth. A. Growth curve depicting the number of EV and HSD10 ov cells measured over seven days demonstrated that HSD10 ov cells grew faster in cell culture. Results displayed as cells × 104 per ml (n = 9). B. Growth curve displaying the number of control shRNA and HSD10 shRNA cells measured over seven days showed that HSD10 shRNA cells grew slower than control shRNA cells. Results depicted as cells × 104 per ml (n = 9). C. EV and HSD10 ov cells were injected into the mammary fat pad tissue of 20 two-month old female SCID mice. Day 30 tumor growth in two SCID mice inoculated with EV (left mouse) or HSD10 ov (right mouse) cells demonstrated considerable tumor growth in the HSD10 tumor mouse when observed beside the EV tumor mouse. Visualization of tumors was performed 24 hours post-injection of 15 nmol 2-DG optical dye with an In-Vivo Multispectral FX PRO imager. Arrows point to tumors. D. Quantification of tumor growth in all SCID mice injected with EV (n = 8) or HSD10 ov (n = 12) cells grown over a total of 32 days, depicted in tumor volume (mm3). Data presented as mean ± SE. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus control group.