Figure 3

Examination of mitochondrial membrane potential in HSD10-transfected cell lines. A. Confocal microscopy demonstrating immunofluorescence staining of mitochondrial membrane potential with TMRM alone (red), Mito Tracker Green alone (green), and these two antigens co-localized (yellow) in EV and HSD10 ov cells. Scale bar in A: 30 μm. B. Quantification of TMRM immunofluorescence staining (depicted in A) displayed as the percentage of intensity of fluorescence (n = 4). C. Confocal microscopy demonstrating immunofluorescence staining of mitochondrial membrane potential with TMRM alone (red), Mito Tracker Green alone (green), and these two antigens co-localized (yellow) in control shRNA and HSD10 shRNA cells. Scale bar in C: 30 μm. D. Quantification of TMRM immunofluorescence staining (depicted in C) displayed as the percentage of intensity of fluorescence (n = 4). Data presented as mean ± SE. *P < 0.05 versus control group.