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Figure 2 | BMC Cancer

Figure 2

From: Overexpression of 17β-hydroxysteroid dehydrogenase type 10 increases pheochromocytoma cell growth and resistance to cell death

Figure 2

Assessment of mitochondrial bioenergetics in HSD10-transfected cell lines. Electron transport chain complex I (A), II (B), III (C), and IV (D) enzyme activities were assessed in EV, HSD10 ov, control shRNA, and HSD10 shRNA cells. Results, displayed as fold increase (n = 5 for each assay), showed that complex IV activity is enhanced in HSD10 ov cells and all ETC. complex activities are decreased in HSD10 shRNA cells. E. Densitometry of citrate synthase enzyme activity (n = 5) showed no change in activity between EV and HSD10 ov cells, and reduced activity in HSD10 shRNA cells. F. Densitometry of ATP activity (n = 6) demonstrated that ATP levels are increased in HSD10 ov cells and diminished in HSD10 shRNA cells. G. Densitometry of MTT reduction (n = 4) exhibited similar reduction of MTT by all HSD10-transfected cell lines. Data presented as mean ± SE. *P < 0.01, **P < 0.001, ***P < 0.0001 versus EV and control shRNA groups.

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BMC Cancer

ISSN: 1471-2407

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