Table 1 Primer sequences for semi-quantitative RT-PCR and qRT-PCR
Gene | Forward primer 5’ to 3’ | Reverse primer 5’ to 3’ | Product size (bp) |
|---|---|---|---|
ITGA3 | AGCGCTACCTGCTCCTGGCT | GGGCAGTGAGTGGGCACAGG | 99 |
ITGA10 | CTGACAGGTCTCTGCTCCCCC | CCATCGCTGTCCACCCCCAA | 120 |
ITGB8 | TGTGTGCTGGGCATGGAGAGTGT | CAGTGCTGGGCTGCTGCTGAA | 100 |
ITGAV | CATCTGTGAGGTCGAAACAGG | TGGAGCATACTCAACAGTCTTTG | 137 |
EPHB4 | TCAATGTCACCACTGACCGAGAGGTA | GGTATTTGACCTCGTAGTCCAGCACA | 141 |
GAPDH | CTGCACCACCAACTGCTTAG | GTCTTCTGGGTGGCAGTGAT | 108 |