Nicotinergic effects on CytC release from BEC mitochondria. The mitochondria freshly isolated from BEC were exposed to 1 μM staurosporine (control) and incubated in triplicate for 45 min at 30 ° C with 10 μM nicotine in the absence or presence of αBtx (1 μM) and/or Mec (50 μM). The mitochondria were pelleted and the CytC concentration was measured in the supernatants, as described in Materials and Methods. Data are mean + SD. Asterisk = p < 0.05 compared to treatment with staurosporine alone, taken as 100%.