Figure 4

Viral overexpression of TK1 enhanced the cytotoxicity of dT-QX. (a) Western blot analysis of TYMP and TK1 level post transduction of Bel-7402 and HL-7702 cells with either control or TK1 pseudo lentiviral particles; (b) Relative percent protein expression of TYMP and TK1 in western blot analysis after normalization with that of GADPH; (c) and (d) Cell viability MTT results after 24 h treatment with dT-QX post viral transduction in Bel-7402 and HL-7702 cells. Each data point in the graphs was the mean of triplicates with SEM. All experiments were independently repeated at least two times (*P < 0.05 as compared to those under the same dT-QX concentration in cells alone).