Figure 3

Silence of TK1 or TYMP expression impacted dT-QX cytotoxicity. (a) Western blot analysis of TYMP and TK1 in Bel-7402 and Hep3B cells at 48 h post siRNA suppression; (b) Relative percent protein expression of TYMP and TK1 in western blot analysis after normalization with that of GADPH; (c) and (d) Cell viability MTT results after 24 h treatment with dT-QX at 48 h post siRNA suppression in Bel-7402 and Hep3B cells. Each data point in the graphs was the mean of triplicates with SEM. All experiments were independently repeated at least two times (*P < 0.05 as compared to those under the same dT-QX concentration in controls).