Figure 2

Relative quantification of predominant splicing events. (A). The chart shows the ratios between peak areas of alternative splicing events and peak areas of the reference full-length transcripts in tumor samples (T) and blood-derived samples (B). *(P < 0.01) (B). Representative example of a cDNA splicing assays showing Δ1Aq, Δ8p, Δ9,10 and, Δ14p peak areas (blue). The assay allowed us to identify transcripts with different combinations of splicing events such as Δ9, 10 + Δ8p. Interestingly, the assay predicts the existence of another splicing event involving ±16 bp (X in the figure).