Figure 5
In vivoefficacy study. (A-B) HB-002.1 treatment even at low dose (5 mg/kg) reached maximal inhibition of tumor growth in Colo-205 s.c. xenograft model (n = 10 for each group) (A) P < 0.0001 versus PBS treatment for tumor volume over time. (B) P = 0.0002 versus PBS for tumor weight at time of sacrifice. Treatment was started when tumor volume reached to 150-200 mm3, twice a week, through i.p. injection. (C-D) In Colo-205 xenograft model (n = 10 for each group), varying amounts of HB-002.1 (1, 3, 5 mg/kg) were tested on effect on tumor growth. Significant tumor growth inhibition was observed in the lowest dose (1 mg/kg, P < 0.0001 for tumor volume and P = 0.0004 for tumor weight versus PBS). (E-F) Therapeutic efficacy of HB-002.1 in A549 xenograft model (n = 10 for each group) was analyzed and compared with that of bevacizumab at doses of 5, 20 mg/kg. HB-002.1 at low dose (5 mg/kg) exhibited superior efficacy than bevacizumab at high dose (20 mg/kg) (TGI% = 78.02 for HB-002.1, versus 67.55 for bevacizumab) (Table 3).