Effects of IGFBP7 recombinant protein on EOC cell lines. A, Western blot analysis showing an alteration of the phophorylation status of ERK and AKT proteins after rIGFBP7 treatment of 1 ng/μl of OV90 neor cell line as determined at different time points following exposure. Total AKT and total ERK served as a loading control. B, Spheroid formation assay with OV90 neor and TOV112D cell lines in the presence or absence of rIGFBP7 (1 and 10 ng/μl). Images are shown at ×20 magnification. C, wound healing assay of OV90 neor and TOV112D in the presence or absence of rIGFBP7 (1 and 5 ng/μl) observed over a period of 6 days. Images are shown at × 10 magnification.