Nrf2 and resistance to Carmustine. U87MG cells were stably transduced with the Precision LentiORF lentiviral particles to accomplish Nrf2 (MW: ~102 kDa) overexpression evidenced by (A) western blotting. The genetic over expression of Nrf2 resulted in increased Nrf2 transcriptional activity as assessed by (B) TransAM Elisa and augmented expression of HO-1 as assessed by western blotting (C). The confocal image (D) illustrates U87MG cells overexpressed with Red Fluoresent protein (RFP) that served as experimental control (scale bar = 200 μm). The U87MG cells stably over expressing either RFP or Nrf2 were subjected to ether vehicle or Carmustine treatment for 18 h and the cellular viability was assessed by (E) MTT reduction assay and the Figure 4D demonstrates the cellular morphology of cells upon Carmustine treatment using bright field microscopy (Scale bar =200 μm). Data are representative of at least three independent trials (n = 3/trial) and are expressed as mean ± SEM. ** p < 0.01, *** p < 0.001 vs. vehicle-treated cultures.