Figure 3

TBHQ and resistance to Carmustine. U87MG cells were treated with either vehicle or TBHQ (30 μM) for 6 h. After respective treatment, the media were removed, cells were replenished with media containing either vehicle or Carmustine at indicated concentrations and incubated for 18 h. The cell viability was measured using (A) MTT reduction assay and (B) flow cytometry analysis. Data from MTT Assay are representative of three independent experiments and are expressed as mean ± SEM. ** p < 0.01, *** p < 0.001 vs. vehicle treated cells. For flow cytometry analysis, the cells after treatments were collected and stained with 7-AAD (y-axis) a marker of cell death and Annexin V (x –axis), a marker of early apoptotic cell death. The percentages of viable cells (C) which are both Annexin V/7-AAD negative are shown. Data are representative of two independent experiments and are expressed as mean ± SEM. *** p < 0.001 vs. Carmustine alone treated cells.