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Figure 1 | BMC Cancer

Figure 1

From: Overexpression of Nrf2 attenuates Carmustine-induced cytotoxicity in U87MG human glioma cells

Figure 1

TBHQ and Nrf2 upregulation. U87MG cells were treated with either vehicle or TBHQ for 6 h and the induction of Nrf2 (MW: ~102 kDa) was quantified using (A) western blotting followed by (B) densitometry analysis. The immunocytochemistry analysis followed by (C) confocal imaging further confirmed induction and nuclear translocation of Nrf2 in glioma cells by TBHQ (scale bar = 50 μm). TBHQ treatment of glioma cells also resulted in the induction of HO-1 (MW : ~31 kDa), one of the Nrf2 regulated molecular targets, as evidenced by (D) western blotting followed by (E) densitometry analysis. Densitometry is expressed as the mean ± SEM from three independent trials and data were analyzed using One-way ANOVA followed by Dunnett’s post-hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001 vs. vehicle-treated cultures).

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