AA005-induced cell death significantly decreases inAIFknockdown cells. (A) SW620 cells were transfected with scrambled negative control (NC) or siRNAs against AIF (A3 or A5); absence of AIF expression was confirmed by western blot analysis, standardized to actin. (B–D)AIF knockdown SW620 cells and controls were treated with or without 1 μM AA005 for 48 h (B), 500 μM MNNG for 8 h (C), and 20 μM camptothecin for 36 h (D). Annexin-V/PI double stained cells and cell death were measured on flow cytometry. All experiments were repeated 3 times with the same results. Results show mean ± S.D. **P < 0.01, versus AA005- or MNNG-treated NC group.