AA005 induces cell death in various cancer cell lines. (A) Chemical structures of annonaceous acetogenin mimic AA005. (B–E) Adherent cells of SW620 (B) and BT549 (C), leukemic cells of U937 (D) and NB4 (E), were treated with AA005 at indicated concentrations for 48 h. Cell death was measured by trypan-blue exclusion assay; each column represents the mean ± S.D. of triplicate runs in an independent experiment. *P < 0.05, **P < 0.01, compared with the vehicle-only control.