Figure 3

Immunohistochemical staining of the VEGFR-1 ligands and pVEGFR-1 in the vasculature of CC tissue. (A) Characteristic endothelial VEGF expression. VEGF positive intratumoral microvascular vessels with predominantly compressed lumina (A1, x 100) and extratumoral microvascular vessels with open lumina (A2, x 100). (B) Characteristic endothelial PlGF expression: Macrovascular vessels with arteriosclerotic changes (B1, H.E., x 40) and PlGF immunopositivity (B2, x 40) as well as altered macrovascular vessels with discontinuous, hypoplastic smooth muscle cell layer (B3, H.E., x 40) and PlGF immunopositivity (B4, x 40). (C) Characteristic endothelial VEGF-B expression: Small and large vessels with VEGF-B immunopositivity (C1, x 100) and capillaries with absent VEGF-B expression (C2, x100). (D) Characteristic endothelial pVEGFR-1^Tyr1048 expression in small intratumoral vessels (x 100). (E) Characteristic endothelial pVEGFR-1^Tyr1213 expression in small intratumoral vessels (x 100).