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Table 1 The probes and quenchers used for real-time polymerase chain reaction

From: The DNA load of six high-risk human papillomavirus types and its association with cervical lesions

Test Viral type Size (bp) Probe Quencher
Reaction 1 HPV-16 78 FAM ZEN/IBFQ
Reaction 2 HPV-18 80 Cy5 IBRQ
HPV-31 78 HEX ZEN/IBFQ
Reaction 3 HPV-33 78 FAM ZEN/IBFQ
HPV-45 76 Cy5 IBRQ
Reaction 4 HPV-58 109 HEX ZEN/IBFQ
HMBS 76 FAM ZEN/IBFQ
  1. Four parallel duplex real-time PCRs were performed per patient. Probe design for each viral type and HMBS was adjusted based on the types included in each reaction.
  2. HPV: human papillomavirus; FAM: 6-carboxyfluorescein; Cy5: FluoroLink mono reactive dye Cy5; HEX: hexachlorofluoresceine; HMBS: hydroxymethylbilane synthase; ZEN/IBFQ: ZEN and Iowa Black FQ; IBRQ: Iowa Black RQ.