The DNA load of six high-risk human papillomavirus types and its association with cervical lesions

BMC Cancer

Table 1 The probes and quenchers used for real-time polymerase chain reaction

Test	Viral type	Size (bp)	Probe	Quencher
Reaction 1	HPV-16	78	FAM	ZEN/IBFQ
Reaction 2	HPV-18	80	Cy5	IBRQ
Reaction 2	HPV-31	78	HEX	ZEN/IBFQ
Reaction 3	HPV-33	78	FAM	ZEN/IBFQ
Reaction 3	HPV-45	76	Cy5	IBRQ
Reaction 4	HPV-58	109	HEX	ZEN/IBFQ
Reaction 4	HMBS	76	FAM	ZEN/IBFQ

Four parallel duplex real-time PCRs were performed per patient. Probe design for each viral type and HMBS was adjusted based on the types included in each reaction.
HPV: human papillomavirus; FAM: 6-carboxyfluorescein; Cy5: FluoroLink mono reactive dye Cy5; HEX: hexachlorofluoresceine; HMBS: hydroxymethylbilane synthase; ZEN/IBFQ: ZEN and Iowa Black FQ; IBRQ: Iowa Black RQ.

ISSN: 1471-2407