Kaplan-Meier survival curves according to AEG-1 and EMT status, and corresponding Receiver Operating Characteristic analysis. (A) The high AEG-1 group correlated with poor survival of lung cancer patients. (B) In the AEG-1-positive group, EMT(+) status showed a poor survival trend. Patients with E-cadherin (−) and Vimentin(+) were evaluated as EMT(+); Conversely, patients with E-cadherin (+) and Vimentin(−) were evaluated as EMT(−). (C) In the AEG-1-negative group, EMT(+) status also showed a poor survival trend. (D) The combined AEG-1 and EMT status had the largest area under the curve compared with AEG-1 level and EMT status, respectively. (E) AEG-1 mRNA was determined by real-time RT-PCR in tumors with metastasis (n = 30) and those without metastasis (n = 23) and levels of AEG-1 mRNA were expressed as (AEG-1/β-actin mRNA ratio). *P < 0.05 versus nonmetastatic tissues.