AEG-1 expression levels were closely correlated with risk of lymph node metastasis in primary cancer lung cancer. (A) H&E staining was used to identify lung cancer patients with or without lymph node metastasis (magnification × 200). (B) AEG-1 regulates EMT in the different stages of lung cancer, including lung cancer patients with or without metastasis, as determined by positron emission tomography/computed tomography. (C) AEG-1 over-expression closely correlates with changes in the EMT marker in clinical specimens from lung cancer patients. Expression levels of AEG-1, E-cadherin, Vimentin, p-GSK-3β (Ser-9), GSK-3β and β-catenin in normal (n = 3) and lung cancer (n = 3) tissues were determined by Western blotting. Densitometry was used to determine relative protein levels, and all proteins were normalized to the levels of GAPDH.