AEG-1 promoted tumor metastasis in vivo. (A) Representative BLI images of mice bearing Slu-01/AEG-1-expressing tumors with metastatic lesions. Mice (n = 15) were imaged six weeks later to determine local tumor growth and metastasis. (B) Number of metastatic nodules or distant metastasis in individual dead mouse bearing con or Slu-01/AEG-1-expressing tumors. (C) AEG-1 overexpression in Slu-01 cells promoted EMT in athymic nude mice in vivo. H&E staining showed primary tumors without detectable metastasis in control mice and the lymph node metastases in mice bearing Slu-01/AEG-1-expressing tumors two weeks after injection (magnification, ×200). IHC showed that up-regulation of AEG-1 resulted in an increased in the expression of Vimentin and weak E-cadherin staining (magnification × 200).