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Figure 1 | BMC Cancer

Figure 1

From: Astrocyte elevated gene-1(AEG-1) induces epithelial-mesenchymal transition in lung cancer through activating Wnt/β-catenin signaling

Figure 1

Lung cancer cell lines showed different AEG-1 expression characteristics and AEG-1 promoted the EMT process. (A) AEG-1 protein expression levels in lung cancer cell lines. NCI-H226 cells expressed a high level of AEG-1; NCl-H460, L-78, A549 and Slu-1 cells expressed a low level of AEG-1. (B) The expression spectrum of mesenchymal and epithelial markers in AEG-1-knockdown cells and pcDNA3.1-AEG-1-transfected cells was analyzed by using the Western blotting method. GAPDH served as a control. (C) Immunofluorescence staining of AEG-1, E-cadherin and Vimentin in NCI-H226 and Slu-01 cell lines (magnification × 100). (D) Knockdown of AEG-1 reversed EMT in vitro. Morphology of NCl-H226 cells transfected with AEG-1 siRNA was observed through phase-contrast microscopy (magnification × 100). Up-regulation of AEG-1 initiated EMT in vitro. Slu-01 cells were transfected with pcDNA3.1-AEG-1 and the morphology was observed through phase-contrast microscopy (magnification × 100). (E) The effect of AEG-1 expression changes on invasion ability. NCI-H226 cells were transfected with AEG-1 siRNA, and Slu-01 cells were transfected with pcDNA3.1-AEG-1. The data represent the mean ± SD of three independent experiments (asterisk; p < 0.01).

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