Figure 6

Resveratrol suppressed E-cadherin expression via inhibiting the transcription of Snail and reducing its binding on the promoter of E-cadherin. (A) LoVo cells treated with TGF-β1 or (and) resveratrol were analyzed for the Snail and E-cadherin mRNA expression by RT-PCR. **P < 0.01, compared with group without treatment of TGF-β1 and resveratrol. (B) LoVo cells trasfected with pcDNA3.1-Snail or pcDNA3.1 plasmid were analyzed for the Snail and E-cadherin mRNA expression by RT-PCR. **P < 0.01, compared with the empty vector group. (C) Whole or cell extracts of different cellular compartments from LoVo cells expressing pcDNA3.1-Snail or pcDNA3.1 were probed for Snail and E-cadherin. **P < 0.01, compared with the empty vector group. (D) The relative E-cadherin promoter activities were detected in LoVo cells treated with TGF-β1 or (and) resveratrol. **P < 0.01, compared with group without treatment of TGF-β1 and resveratrol. (E) The relative E-cadherin promoter activities were detected in LoVo cells expressing pcDNA3.1-Snail or pcDNA3.1. **P < 0.01, compared with the empty vector group.