Figure 3

Resveratrol inhibited the morphological changes of TGF-β1-induced EMT. (A) LoVo cells were treated with 10 ng/ml TGF-β1 to induce EMT, and resveratrol with a concentration of 6, 12 μM were introduced to inhibit the morphological changes. Control LoVo cells displayed classical epithelial morphology, and 10 ng/ml TGF-β1 treated LoVo cells represented a mesenchymal phenotype. (B) Expression of epithelial phenotype marker E-cadherin and mesenchymal phenotype marker Vimentin were detected by western blot, **P < 0.01, compared with control LoVo cells without treatment of TGF-β1 and resveratrol. (C) Immunofluorescence staining of E-cadherin and Vimentin in LoVo cells treated with 10 ng/ml TGF-β1, with or without resveratrol.