Table 1 Real-time PCR primer sequences
From: Nuclear factor I-C regulates E-cadherin via control of KLF4 in breast cancer
Gene name | Â | Primer |
|---|---|---|
hNFI-C | forward | 5′-CGA CTT CCA GGA GAG CTT TG-3′ |
reverse | 5′-GTT CAG GTC GTA TGC CAG GT-3′ | |
hKLF4 | forward | 5′-CCC ACA CAG GTG AGA AAC CT-3′ |
reverse | 5′-TTC TGG CAG TGT GGG TCA TA-3′ | |
hSlug | forward | 5′-GAG CAT TTG CAG ACA GGT CA-3′ |
reverse | 5′-CCT CAT GTT TGT GCA GGA GA-3′ | |
hE-cadherin | forward | 5′-TGC CCA GAA AAT GAA AAA GG-3′ |
reverse | 5′-GTG TAT GTG GCA ATG CGT TC-3′ | |
hVimentin | forward | 5′-AAA GCG TGG CTG CCA AGA AC-3′ |
reverse | 5′-GTG ACT GCA CCT GTC TCC GGT A-3′ | |
hN-cadherin | forward | 5′-CGA ATG GAT GAA AGA CCC ATC C-3′ |
reverse | 5′-GGA GCC ACT GCC TTC ATA GTC AA-3′ | |
hP-cadherin | forward | 5′-GCA GAA GTC AGC GAG AAA GGA G-3′ |
reverse | 5′-GGA GGA TGA AAC CAC CCT TCC A-3′ | |
hGAPDH | forward | 5′- CCA TGG AGA AGG CTG GGG-3′ |
reverse | 5′- CAA AGT TCT CAT GGA TGA CC-3′ |